CCEH centralized resource site for sharing resources and protocols.

Protocols

Center

Core

Title

TitleCenterCore FacilityLinkhf:tax:cceh-centerhf:tax:media-type
Analysis and Interpretation of single cells sequencing data – part 1 Introduction and alignment
medicine.yale.edu/labmed/ycceh/media-player/analysis-and-interpretation-of-single-cell-sequencing-data/yalevideo
Analysis and Interpretation of single cells sequencing data – part 2
medicine.yale.edu/labmed/ycceh/media-player/scrna-sequencing-data-processing/yalevideo
Analysis and Interpretation of single cells sequencing data – part 3 Key Analysis Steps
medicine.yale.edu/labmed/ycceh/media-player/key-analysis-steps/yalevideo
Analysis and Interpretation of single cells sequencing data – part 4 Multiple Test Correction
medicine.yale.edu/labmed/ycceh/media-player/multiple-test-correction/yalevideo
Bone Marrow Harvesting
Hypoxia Coreutah-a3-prod.s3.us-west-2.amazonaws.com/ckuwte8u10009o5pwplegadp4/attachments/ckzyddoam003l4mpwxv65efxy-hypoxia-core-sop-bm-harvest-080221.pdfindianapdf
Bone Marrow Transplant
Hypoxia Coreutah-a3-prod.s3.us-west-2.amazonaws.com/ckuwte8u10009o5pwplegadp4/attachments/ckzydco58003g4mpw54dn38u9-hypoxia-core-sop-bm-transplant-080221.pdfindianapdf
CD34 Selection Procedure
Fred Hutch Hematopoietic Cell Procurement and Resource Developmentutah-a3-prod.s3.us-west-2.amazonaws.com/ckuwte8u10009o5pwplegadp4/attachments/ckzydr8100034e7pvcq76b6g1-cd34-selection-procedure.docxfred-hutchdoc
Cell Thawing Instructions
Fred Hutch Hematopoietic Cell Procurement and Resource Developmentutah-a3-prod.s3.us-west-2.amazonaws.com/ckuwte8u10009o5pwplegadp4/attachments/cl6n7s3eh00jd0vmjcdjh9ue7-cell-thawing-instructions.docfred-hutchdoc
Colony Assay
Hypoxia Coreutah-a3-prod.s3.us-west-2.amazonaws.com/ckuwte8u10009o5pwplegadp4/attachments/ckzydfixz002xe7pvdl7tpr3f-hypoxia-core-sop-colony-assay-080221.pdfindianapdf
Determining Mouse Body Condition Score
utah-a3-prod.s3.us-west-2.amazonaws.com/ckuwte8u10009o5pwplegadp4/attachments/clv2tbt1k1hw10nll99ux8vod-body-condition-scoring.pdfindianapdf
Enriching Rare Populations of Human CD34 Cells by FACS
medicine.yale.edu/labmed/ycceh/media-player/enriching-rare-populations-cd34-cells-facs/yalevideo
Expansion and Differentiation of Human Erythroid Populations from CD34+ Cells
utah-a3-prod.s3.us-west-2.amazonaws.com/ckuwte8u10009o5pwplegadp4/attachments/clzubxdep12d40nnx7kgkfr3j-ycceh-protocol-human-erythroid-expansion-from-cd34-413537-51384-v2.pdfyalepdf
Flow Cytometry Sample Staining
Fred Hutch Hematopoietic Cell Procurement and Resource Developmentutah-a3-prod.s3.us-west-2.amazonaws.com/ckuwte8u10009o5pwplegadp4/attachments/ckzycv9xy001v4mpw6oytvtw7-flow-cytometry-sample-staining.docxfred-hutchdoc
Flow Staining
Hypoxia Coreutah-a3-prod.s3.us-west-2.amazonaws.com/ckuwte8u10009o5pwplegadp4/attachments/ckzydb9jv002je7pvg6rl3o7l-hypoxia-core-sop-flow-staining.pdfindianapdf
Guidelines for Agent Administration
utah-a3-prod.s3.us-west-2.amazonaws.com/ckuwte8u10009o5pwplegadp4/attachments/clv2td9do1i370np14zs0hcze-guidelines-for-agent-administration.pdfindianapdf
Guidelines for Blood Collection
utah-a3-prod.s3.us-west-2.amazonaws.com/ckuwte8u10009o5pwplegadp4/attachments/clv2tf9q11i570np17s5nh5rz-guidelines-for-blood-collection.pdfindianapdf
Indiana U54 Experimental Mouse Core Protocols
medicine.iu.edu/research-centers/nonmalignant-hematology/experimental-mouse-resourcesindianawebsite
Indiana U54 Flow Cytometry Core Protocols
medicine.iu.edu/research-centers/nonmalignant-hematology/flow-tissue-cytometryindianawebsite
Iron and Heme
Iron and Heme Core Facilitybookstack.cores.utah.edu/books/protocolsutahwebsite
IVT Core SOP IP Drug Administration
utah-a3-prod.s3.us-west-2.amazonaws.com/ckuwte8u10009o5pwplegadp4/attachments/clv2ti6u11i1w0nlldqi98vrw-ivt-core-sop-ip-drug-administration.pdfindianapdf
IVT Core SOP SQ Drug Administration
utah-a3-prod.s3.us-west-2.amazonaws.com/ckuwte8u10009o5pwplegadp4/attachments/clv2th6l81i100nllf0ka7zvf-ivt-core-sop-sq-drug-administration.pdfindianapdf
IVT SOP IV Drug Administration
utah-a3-prod.s3.us-west-2.amazonaws.com/ckuwte8u10009o5pwplegadp4/attachments/clv2tklb11ial0np158vzbixi-ivt-sop-iv-drug-administration.pdfindianapdf
Lentiviral Preparation (Calcium Phosphate Method)
utah-a3-prod.s3.us-west-2.amazonaws.com/ckuwte8u10009o5pwplegadp4/attachments/clzubswkj11xn0np50g7jg6az-ycceh-protocol-lentivirus-preparation-calcium-phosphate-413538-51384-v2.pdfyalepdf
Metabolomics Core Protocols
Metabolomics Core Facilitybookstack.cores.utah.edu/shelves/metabolomicsutahwebsite
Mutation Generation and Detection Core Protocols
Mutation Generation & Detectioncores.utah.edu/mutation-generation-and-detection/utahwebsite
Oral administration of Test Material including gavage and additives to drinking water or food
utah-a3-prod.s3.us-west-2.amazonaws.com/ckuwte8u10009o5pwplegadp4/attachments/clv2tg6ww1i690np1he0m03ic-ivt-core-sop-oral-drug-administration.pdfindianapdf
Polysome Preparation from Eukaryotic Cells
utah-a3-prod.s3.us-west-2.amazonaws.com/ckuwte8u10009o5pwplegadp4/attachments/clzubvfst12cn0nnx0k71b2tq-ycceh-protocol-polysome-preparation-413539-51384-v2.pdfyalepdf
Procedure for Cryopreservation of Cells in Bags
Fred Hutch Hematopoietic Cell Procurement and Resource Developmentutah-a3-prod.s3.us-west-2.amazonaws.com/ckuwte8u10009o5pwplegadp4/attachments/ckzyc6m3h001h4mpwogwtzg48-procedure-for-cryopreservation-of-cells-in-bags.docxfred-hutchdoc
Procedure for Making Freeze Solution
Fred Hutch Hematopoietic Cell Procurement and Resource Developmentutah-a3-prod.s3.us-west-2.amazonaws.com/ckuwte8u10009o5pwplegadp4/attachments/ckwuxqhph000afo1xsb5zuwi4-procedure-for-making-freeze-solution.docxfred-hutchdoc
Procedure for Obtaining Samples From Tubes and Bags
Fred Hutch Hematopoietic Cell Procurement and Resource Developmentutah-a3-prod.s3.us-west-2.amazonaws.com/ckuwte8u10009o5pwplegadp4/attachments/ckwpc3f7s000acc1xro3mc12g-procedure-for-obtaining-samples-from-tubes-and-bags.docxfred-hutchdoc
Retrovirus Production (Lipofectamine 3000 Method)
utah-a3-prod.s3.us-west-2.amazonaws.com/ckuwte8u10009o5pwplegadp4/attachments/clzuc0opk12ea0nnxgzfh92ak-ycceh-protocol-retrovirus-production-lipofectamine3000-method-413540-51384-v2.pdfyalepdf
Simplified Procedure for Crypreserving Cells in Cryovials
Fred Hutch Hematopoietic Cell Procurement and Resource Developmentutah-a3-prod.s3.us-west-2.amazonaws.com/ckuwte8u10009o5pwplegadp4/attachments/ckzyctp340017e7pvlkp4s8u1-simplified-procedure-for-crypreserving-cells-in-cryovials.docxfred-hutchdoc
Using the Cryomed Freezer for Cryopreservation of Samples
Fred Hutch Hematopoietic Cell Procurement and Resource Developmentutah-a3-prod.s3.us-west-2.amazonaws.com/ckuwte8u10009o5pwplegadp4/attachments/ckzycwfv3001ie7pvwrcq7d0a-using-the-cryomed-freezer-for-cryopreservation-of-samples.docxfred-hutchdoc
Virus Infection
utah-a3-prod.s3.us-west-2.amazonaws.com/ckuwte8u10009o5pwplegadp4/attachments/clzubz8pn11zi0np59zdx4zgb-ycceh-protocol-virus-infection-418265-51384-v1.pdfyalepdf