Fred Hutchinson Cancer Center

https://www.fredhutch.org/en/research/divisions/clinical-research-division/research/co-operative-center-for-excellence-in-hematology.html

 

Providing the cellular resources for the future advancements in hematopoietic stem biology. 

Fred Hutch’s unique resources include enriched hematopoietic progenitor/stem cell products coupled with platform of molecular tools and large animal models to investigate hematopoietic stem cell biology. 

  • The¬†Hematopoietic Cell Procurement and Processing Core¬†provides an array of unmanipulated and enriched subpopulations of hematopoietic cell products from healthy donors, including CD34+ hematopoietic progenitor/stem cells, to investigators.

  • The¬†Cell Tools and Manipulation Core¬†designs and provides a range of different tools for investigators to manipulate and edit hematopoietic blood and progenitor/stem cells including Lentivirus, Adeno-Associated Virus (AAV), Gamma retrovirus, and Foamy Virus at scales for¬†ex vivo¬†and¬†in vivo¬†experiments.¬† The Core also offers the development of user-directed, edited CD34+ hematopoietic progenitor/stem cells upon request.

  • The¬†Large Animal Cell and Molecular Services Core¬†provides Chimerism analysis, DLA Typing, design of molecular screening methods, and clone tracking for large animal models

  • Enrichment Core¬†introduces the exciting world of research to a demographically diverse population of high school students, to aid in the development of the next generation of investigators.


Cores

Protocols

Center Title subCategory Link

Fred Hutchinson Cancer Center

Cell Thawing Instructions

Fred Hutchinson Cancer Center

CD34 Selection Procedure

Fred Hutchinson Cancer Center

Using the Cryomed Freezer for Cryopreservation of Samples

Fred Hutchinson Cancer Center

Flow Cytometry Sample Staining

Fred Hutchinson Cancer Center

Simplified Procedure for Crypreserving Cells in Cryovials

Fred Hutchinson Cancer Center

Procedure for Making Freeze Solution

Fred Hutchinson Cancer Center

Procedure for Obtaining Samples From Tubes and Bags

Fred Hutchinson Cancer Center

Procedure for Cryopreservation of Cells in Bags