Fred Hutchinson Cancer Center
https://www.fredhutch.org/en/research/divisions/clinical-research-division/research/co-operative-center-for-excellence-in-hematology.html
Providing the cellular resources for the future advancements in hematopoietic stem biology.
Fred Hutch’s unique resources include enriched hematopoietic progenitor/stem cell products coupled with platform of molecular tools and large animal models to investigate hematopoietic stem cell biology.
- The Hematopoietic Cell Procurement and Processing Core provides an array of unmanipulated and enriched subpopulations of hematopoietic cell products from healthy donors, including CD34+ hematopoietic progenitor/stem cells, to investigators.
- The Cell Tools and Manipulation Core designs and provides a range of different tools for investigators to manipulate and edit hematopoietic blood and progenitor/stem cells including Lentivirus, Adeno-Associated Virus (AAV), Gamma retrovirus, and Foamy Virus at scales for ex vivo and in vivo experiments. The Core also offers the development of user-directed, edited CD34+ hematopoietic progenitor/stem cells upon request.
- The Large Animal Cell and Molecular Services Core provides Chimerism analysis, DLA Typing, design of molecular screening methods, and clone tracking for large animal models
- Enrichment Core introduces the exciting world of research to a demographically diverse population of high school students, to aid in the development of the next generation of investigators.
Cores
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Cell Manipulation ToolsThe Cell Manipulation Tools Core offers a range of solutions, providing tools to silence or introduce genes of interest into cells. We offer both CRISPR-Cas9 based genome editing as well as full service viral preparation services.https://www.fredhutch.org/en/research/divisions/clinical-research-division/research/co-operative-center-for-excellence-in-hematology/cell-manipulation-tools.html
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Hematopoietic Cell Procurement and Resource DevelopmentProviding Highly-Defined Cell Populations to Further Hematologic Researchhttps://www.fredhutch.org/en/research/divisions/clinical-research-division/research/co-operative-center-for-excellence-in-hematology/hematopoietic-cell-procurement-and-resource-development.html
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Enrichment CoreWe offer paid internships and introductory programs for high school students throughout our organization. Each program offers valuable learning opportunities that support academic and career goals.https://www.fredhutch.org/en/research/divisions/clinical-research-division/research/co-operative-center-for-excellence-in-hematology.html
Protocols
- Cell Thawing Instructions
- CD34 Selection Procedure
- Using the Cryomed Freezer for Cryopreservation of Samples
- Flow Cytometry Sample Staining
- Simplified Procedure for Crypreserving Cells in Cryovials
- Procedure for Making Freeze Solution
- Procedure for Obtaining Samples From Tubes and Bags
- Procedure for Cryopreservation of Cells in Bags