Fred Hutchinson Cancer Center
https://www.fredhutch.org/en/research/divisions/clinical-research-division/research/co-operative-center-for-excellence-in-hematology.html
Providing the cellular resources for the future advancements in hematopoietic stem biology.
Fred Hutch’s unique resources include enriched hematopoietic progenitor/stem cell products coupled with platform of molecular tools and large animal models to investigate hematopoietic stem cell biology.
- The Hematopoietic Cell Procurement and Processing Core provides an array of unmanipulated and enriched subpopulations of hematopoietic cell products from healthy donors, including CD34+ hematopoietic progenitor/stem cells, to investigators.
- The Cell Tools and Manipulation Core designs and provides a range of different tools for investigators to manipulate and edit hematopoietic blood and progenitor/stem cells including Lentivirus, Adeno-Associated Virus (AAV), Gamma retrovirus, and Foamy Virus at scales for ex vivo and in vivo experiments. The Core also offers the development of user-directed, edited CD34+ hematopoietic progenitor/stem cells upon request.
- The Large Animal Cell and Molecular Services Core provides Chimerism analysis, DLA Typing, design of molecular screening methods, and clone tracking for large animal models
- Enrichment Core introduces the exciting world of research to a demographically diverse population of high school students, to aid in the development of the next generation of investigators.
Cores
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Cell Manipulation ToolsThe Cell Manipulation Tools Core offers a range of solutions, providing tools to silence or introduce genes of interest into cells. We offer both CRISPR-Cas9 based genome editing as well as full service viral preparation services.https://www.fredhutch.org/en/research/divisions/clinical-research-division/research/co-operative-center-for-excellence-in-hematology/cell-manipulation-tools.html
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Hematopoietic Cell Procurement and Resource DevelopmentProviding Highly-Defined Cell Populations to Further Hematologic Researchhttps://www.fredhutch.org/en/research/divisions/clinical-research-division/research/co-operative-center-for-excellence-in-hematology/hematopoietic-cell-procurement-and-resource-development.html
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Enrichment CoreWe offer paid internships and introductory programs for high school students throughout our organization. Each program offers valuable learning opportunities that support academic and career goals.https://www.fredhutch.org/en/research/divisions/clinical-research-division/research/co-operative-center-for-excellence-in-hematology.html
Protocols
CD34 Selection Procedure
Cell Thawing Instructions
Flow Cytometry Sample Staining
Procedure for Cryopreservation of Cells in Bags
Procedure for Making Freeze Solution
Procedure for Obtaining Samples From Tubes and Bags
Simplified Procedure for Crypreserving Cells in Cryovials
Using the Cryomed Freezer for Cryopreservation of Samples