Expansion and Differentiation of Human Erythroid Populations from CD34+ Cells

Contributor - Yale University School of Medicine

This protocol uses CD34+ cells obtained from YCCEH Cell Prep and Analysis Core (typically available as vials each with 1 million cells) to generate erythroid cells in vitro. Cells are isolated from mobilized peripheral blood mononuclear cells (PBMC) from normal donors. Other sources of starting cells including CD34+ cells (from bone marrow or cord blood) can be used. Unfractionated peripheral blood, bone marrow or mobilized PBMC are also used in some protocols.