Yale – CCEH

Yale Cooperative Center of Excellence in Hematology

https://medicine.yale.edu/labmed/ycceh/

Hematologic Single cell functional, molecular, spatial and morphological characterization of stem cells.

The YCCEH applies innovative, emerging technologies to study normal and perturbed hematopoiesis.

The Cell Preparation and Analysis Core provides training for and performance of a wide range of hematology-related services and techniques including cutting-edge single cell functional studies with and without cell indexing.
The Imaging Core brings education and access in all aspects of microscopy. The YCCEH is unique in providing technical expertise in super-resolution microscopy including expansion microscopy and development of improved functional readouts of human progenitor cell fate specification including timelapse microscopy.
The Animal Modeling Core provides access to the latest technologies for hematologic studies in animal models, including xenogeneic transplantation into highly humanized immunodeficient mice allowing for multilineage engraftment of functional human blood cell types in highly humanized mouse models.
The Enrichment Core promotes hematology research-related scientific exchange between trainees and investigators. This includes the Rising Stars in Hematology Seminar Series.

Cores

Animal Modeling Core

Need to generate a mouse model for your hematology research? Check out the Yale Animal Modeling Core sponsored by the Cooperative Center of Excellence in Hematology.

https://medicine.yale.edu/labmed/ycceh/core_d/
Cell Preparation and Analysis Core

The Core will provide free training and cost-recovery-based services for in vitro assays of hematopoietic cells including liquid cultures for differentiation down specific hematopoietic lineages as well as clonogenic assays in semisolid medium.

https://medicine.yale.edu/labmed/ycceh/cell-prep-analysis-core/
Imaging Core

https://medicine.yale.edu/labmed/ycceh/imaging-core/
Enrichment Core

https://medicine.yale.edu/labmed/ycceh/enrichment/

Analysis and Interpretation of single cells sequencing data – part 1 Introduction and alignment

Analysis and Interpretation of single cells sequencing data – part 2

Analysis and Interpretation of single cells sequencing data – part 3 Key Analysis Steps

Analysis and Interpretation of single cells sequencing data – part 4 Multiple Test Correction

Enriching Rare Populations of Human CD34 Cells by FACS

Enriching Rare Populations of Primary Human CD34+ Cells by FACS

Expansion and Differentiation of Human Erythroid Populations from CD34+ Cells

Lentiviral Preparation (Calcium Phosphate Method)

Polysome Preparation from Eukaryotic Cells

Retrovirus Production (Lipofectamine 3000 Method)

Virus Infection