Fred Hutchinson Cancer Research Center

The CCEH Administrative Core provides scientific and budgetary oversight for all CCEH activities. This includes, in addition to the Pilot and Feasibility Program, an Enrichment Program that supports outside speakers, high school summer internships, and short-term on-sight training opportunities for NIDDK-funded trainees from anywhere in the US. The Administrative Core also houses a self-supporting computer graphics specialist and poster printing services.

Protocols

CD34 Selection Procedure

Fred Hutchinson Cancer Research Center
This procedure is a general method to obtain and select for the CD34+ cell compartment. It uses antibody coated immunomagnetic beads to select for those cells. It is important to follow all biohazard safety precautions when performing this procedure. CD34+ selections that we conduct use current Miltenyi processing procedures.
Download

Flow Cytometry Sample Staining

Fred Hutchinson Cancer Research Center
The purpose of this procedure is to create samples for flow cytometry enumeration. This method if used with sterile materials and under sterile technique conditions can be also used for cell sorting. For this procedure use biohazard safety precautions at all times.
Download

Procedure for Cryopreservation of Cells in Bags

Fred Hutchinson Cancer Research Center
This procedure is used for cryopreserving large aliquots of cells in cryo storage bags. This procedure is for cell numbers greater than 1-2 billion cells. It is important to follow all biohazard safety precautions when using this procedure. STERILE TECHNIQUE SHALL BE FOLLOWED AT ALL TIMES USING THIS PROCEDURE
Download

Procedure for Making Freeze Solution

Fred Hutchinson Cancer Research Center
Cells require the correct amount of DMSO and nutrient for sustainability when cryopreserved and thawed. STERILE TECHNIQUE SHALL BE USED FOR PREPARATION OF THIS SOLUTION
Download

Procedure for Obtaining Samples From Tubes and Bags

Fred Hutchinson Cancer Research Center
The purpose of this procedure is to obtain a small aliquot from either a tube of blood or larger bag of cells. The small aliquot is to be a representative sample of the main body of cells contained in the tube or bag. This aliquot can then be used for cell counts or flow cytometry staining. The information garnered from the aliquot can then be extrapolated to the larger source. For this procedure use biohazard precautions.
Download